CD5 antibody (CF®488A)

Catalog No. ABIN6185074

The Mouse Monoclonal anti-CD5 antibody has been validated for FACS, IF and IP. It is suitable to detect CD5 in samples from Human and Rhesus Monkey.

Quick Overview for CD5 antibody (CF®488A) (ABIN6185074)

Target: CD5

Reactivity: Human, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: Cris-1

Product Details anti-CD5 Antibody (CF®488A)

Purpose: Mouse Monoclonal anti-CD5 (Cris-1), CF488A Conjugate

Characteristics: This antibody recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I, WS Code T 29HLDA III, WS Code T 530). The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Optimal dilution for a specific application should be determined by user

Comment:

293T, Ramos, or MOLT-4 Cells. Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

Molecular Weight: 67 kDa

Gene ID: 921, 58685

UniProt: P06127