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CD5 antibody (CF®594)

This Mouse Monoclonal antibody specifically detects CD5 in FACS, IF and IP. It exhibits reactivity toward Human and Rhesus Monkey.
Catalog No. ABIN6185076

Quick Overview for CD5 antibody (CF®594) (ABIN6185076)

Target

See all CD5 Antibodies
CD5

Reactivity

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Human, Rhesus Monkey

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD5 antibody is conjugated to CF®594

Application

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone

Cris-1
  • Purpose

    Mouse Monoclonal anti-CD5 (Cris-1), CF594 Conjugate

    Characteristics

    This antibody recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I, WS Code T 29HLDA III, WS Code T 530). The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    Stimulated human leukocytes

    Isotype

    IgG2a kappa
  • Application Notes

    Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
    • Optimal dilution for a specific application should be determined by user

    Comment

    293T, Ramos, or MOLT-4 Cells. Tonsil.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from light
  • Target

    CD5

    Alternative Name

    CD5

    Background

    CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

    Molecular Weight

    67 kDa

    Gene ID

    921, 58685

    UniProt

    P06127
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