CD70 antibody (CF®640R)

Catalog No. ABIN6185094

This Mouse Monoclonal antibody specifically detects CD70 in ELISA, FACS, IP, IF, IHC (fro), Coat and ProA. It exhibits reactivity toward Human.

Quick Overview for CD70 antibody (CF®640R) (ABIN6185094)

Target: CD70 (CD70 Molecule (CD70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD70 antibody is conjugated to CF®640R

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Coating (Coat), Proliferation Assay (ProA)

Clone: BU69

Product Details anti-CD70 Antibody (CF®640R)

Purpose: Mouse Monoclonal anti-CD70 (BU69), CF640R Conjugate

Characteristics: This antibody recognizes a protein of 30 kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (frozen) 0.5-1 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• ELISA For coating, order Ab without BSA
• Inhibits T cell proliferation induced by dendritic cells
• Optimal dilution for a specific application should be determined by user

Comment:

Raji cells. Lymph node or tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for CD70

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD70

Background: CD27 ligand (CD27L), CD27LG, Ki24, Surface antigen CD70, Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

Molecular Weight: ~30 kDa

Gene ID: 970

UniProt: P32970

Pathways: Cancer Immune Checkpoints