ETS1 antibody (AA 137-230)

Catalog No. ABIN6187633

The Mouse Monoclonal anti-ETS1 antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect ETS1 in samples from Human.

Quick Overview for ETS1 antibody (AA 137-230) (ABIN6187633)

Target: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ETS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ETS1-1282

Product Details anti-ETS1 Antibody

Binding Specificity: AA 137-230

Purpose: Mouse Monoclonal anti-ETS1-/ (ETS1/1282), Purified, with BSA

Characteristics: ETS1 proto-oncogene is an important transcription factor that plays a role in cell proliferation and differentiation. ETS1 is related to the growth of carcinoma cells by its regulation of the transcription of matrix metalloproteinases and urokinase-type plasminogen activator. The processes of tumor invasion and metastasis depend on the increased proteolytic activity of the invading tumor cells that may involve matrix metalloproteinases, cathepsins B and D and plasminogen activator in the metastatic cascade. ETS1 is preferentially expressed in lymphoid cells, where it is essential for the maintenance of the normal pool of resting T and B cells. ETS1 expression level and distribution are differentially controlled in resting, activated and apoptotic lymphocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human ETS1 fragment (aa137-230) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 1-2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Western blotting 1-2 μg/mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 and HeLa cells. Tonsil, Skin, Pancreas, Kidney and Lymphoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for ETS1

Target: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Alternative Name: ETS1-

Background: Avian erythroblastosis virus E26 (v ets) oncogene homolog 1, ETS1, EWSR2, p54, v-ets erythroblastosis virus E26 oncogene homolog 1

Molecular Weight: 54 kDa

Gene ID: 2113, 369438

UniProt: P14921