pan Keratin antibody (Biotin)

Catalog No. ABIN6190211

This Mouse Monoclonal antibody specifically detects pan Keratin in IF, FACS and WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Monkey, Pig, Frog, Guinea Pig and Goat.

Quick Overview for pan Keratin antibody (Biotin) (ABIN6190211)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Monkey, Pig, Frog, Guinea Pig, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Clone: KRT-457

Product Details anti-pan Keratin Antibody (Biotin)

Purpose: Mouse Monoclonal anti-Cytokeratin, multi (KRT/457), Biotin Conjugate

Characteristics: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 0.5-1.0 μg/mL
• Western blotting 0.5-1.0 μg/mL

Comment:

A431 cells, Skin, Colon carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Cytokeratin, multi

Background: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Molecular Weight: Multiple

Gene ID: 3851, 3852, 3853