pan Keratin antibody (CF®647)

Catalog No. ABIN6190228

This anti-pan Keratin antibody is a Mouse Monoclonal antibody detecting pan Keratin in IF, FACS, WB and IHC (f). Suitable for Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit and Chicken.

Quick Overview for pan Keratin antibody (CF®647) (ABIN6190228)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: AE-1-AE-3

Product Details anti-pan Keratin Antibody (CF®647)

Purpose: Mouse Monoclonal anti-Cytokeratin, pan (AE-1 / AE-3), CF647 Conjugate

Characteristics: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human epidermal keratin

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Skin, Adeno- or Squamous carcinomas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Cytokeratin, pan

Background: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Molecular Weight: 40-67 kDa

Gene ID: 374454, 51350