Nuclear Antigen antibody

Catalog No. ABIN6190865

This Mouse Monoclonal antibody specifically detects in IF, ICC, FACS and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Nuclear Antigen antibody (ABIN6190865)

Target: Nuclear Antigen

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: NM106

Product Details

Purpose: Mouse Monoclonal anti-Human Nuclear Antigen (NM106), Purified, BSA-free

Characteristics: This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Nuclei of HL60 cells

Isotype: IgG1 kappa

Application Details

Application Notes: Does not react with pig, others not known

• Immunohistology formalin-paraffin 0.25-0.5 μg/mL
• Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL

Comment:

All human cells. Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details

Target: Nuclear Antigen

Background: Nuclear Antigen