HRPT2 antibody (AA 127-161)

Catalog No. ABIN6243752

This Rabbit Polyclonal antibody specifically detects HRPT2 in WB. It exhibits reactivity toward Human.

Quick Overview for HRPT2 antibody (AA 127-161) (ABIN6243752)

Target: HRPT2 (CDC73) (Parafibromin (CDC73))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRPT2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB30818

Product Details anti-HRPT2 Antibody

Binding Specificity: AA 127-161

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CDC73 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human CDC73.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for HRPT2

Target: HRPT2 (CDC73) (Parafibromin (CDC73))

Alternative Name: CDC73

Background: Tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. May be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression. Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the cleavage and polyadenylation specificity factor (CPSF) complex and the cleavage stimulation factor (CSTF) complex, and with Wnt signaling. Involved in polyadenylation of mRNA precursors.

Molecular Weight: 60577

UniProt: Q6P1J9

Pathways: Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance