PLK4 antibody (AA 724-758)

Catalog No. ABIN6243950

This Rabbit Polyclonal antibody specifically detects PLK4 in WB. It exhibits reactivity toward Human.

Quick Overview for PLK4 antibody (AA 724-758) (ABIN6243950)

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB53118

Product Details anti-PLK4 Antibody

Binding Specificity: AA 724-758

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PLK4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 724-758 amino acids from the region of human PLK4.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for PLK4

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Alternative Name: PLK4

Target Type: Phage Protein

Background: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.

Molecular Weight: 108972

UniProt: O00444

Pathways: M Phase