ADIPOQ antibody

Catalog No. ABIN6251811

This Mouse Monoclonal antibody specifically detects ADIPOQ in ELISA, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for ADIPOQ antibody (ABIN6251811)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADIPOQ antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ADPQ-1

Product Details anti-ADIPOQ Antibody

Purification: Protein G affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for this Adiponectin antibody.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

ELISA: order BSA free format for coating

IHC (FFPE): 1-2 μg/mL (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Adiponectin antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: Adiponectin

Background: Adiponectin (commonly called ADIPO, or by the gene name ADIPOQ) is an adipocytokine, a hormone produced in adipose tissue. It is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma ADIPO levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women, levels tend to be higher than in men, which may be due to androgens suppressing ADIPO levels. Furthermore, adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low ADIPO levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.

Gene ID: 9370

UniProt: Q15848