Integrin beta 4 antibody

Catalog No. ABIN6251855

This anti-Integrin beta 4 antibody is a Mouse Monoclonal antibody detecting Integrin beta 4 in WB, FACS, IF and BI. Suitable for Human.

Quick Overview for Integrin beta 4 antibody (ABIN6251855)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI)

Clone: UM-A9

Product Details anti-Integrin beta 4 Antibody

Purification: Protein G affinity chromatography

Immunogen: The human squamous cell carcinoma line (UM-SCC1) was used as the immunogen for this Integrin beta-4 / CD104 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Functional Blocking: partially blocks laminin binding, order the BSA/azide free format

Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: Integrin Beta-4

Background: This antibody recognizes a protein of 205  kDa, which is identified as integrin beta 4 (ITGB4) and CD104. Its epitope is localized in the extracellular domain of integrin beta 4 protein. Integrins are heterodimers comprised of < font face=symbol>a and < font face=symbol>b subunits, that are non-covalently associated transmembrane glycoprotein receptors. Different combinations of < font face=symbol>a and < font face=symbol>b polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha-6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex