CD31 antibody

Catalog No. ABIN6252198

This Mouse Monoclonal antibody specifically detects CD31 in FACS, WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for CD31 antibody (ABIN6252198)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: PCM25-1

Product Details anti-CD31 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for this CD31 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/10e6 cells

IF: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the CD31 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background: CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization