CD16 antibody

Catalog No. ABIN6252332

This anti-CD16 antibody is a Mouse Monoclonal antibody detecting CD16 in FACS, IF and Func. Suitable for Human.

Quick Overview for CD16 antibody (ABIN6252332)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: HO-80

Product Details anti-CD16 Antibody

Purification: Protein G affinity chromatography

Immunogen: PBL s from a NK-leukemia patient were used as the immunogen for the CD16 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Functional Activity (Order SAF formulation)

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Optimal dilution of the CD16 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: It recognizes CD16 (Fcgamma,RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80  kDa. Two similar genes represent CD16, CD16A (Fcgamma,RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma,RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Gene ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process