CD16 antibody

Catalog No. ABIN6252333

This Mouse Monoclonal antibody specifically detects CD16 in FACS, IF, WB and Func. It exhibits reactivity toward Human.

Quick Overview for CD16 antibody (ABIN6252333)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Functional Studies (Func)

Clone: HuNK2

Product Details anti-CD16 Antibody

Purification: Protein G affinity chromatography

Immunogen: Human peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Functional Activity (Order SAF formulation)

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Optimal dilution of the CD16 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: Recognizes a protein of 50-65  kDa, identified as CD16 (Workshop IV, Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.

Gene ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process