GJB1 antibody

Catalog No. ABIN6252465

This Rat Monoclonal antibody specifically detects GJB1 in WB, IF, FACS and IHC (fro). It exhibits reactivity toward Rat and Mouse.

Quick Overview for GJB1 antibody (ABIN6252465)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Rat, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: R5-21C

Product Details anti-GJB1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (Frozen): 1-2 μg/mL for 30 min at RT

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: Connexin 32

Background: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Gene ID: 14618

UniProt: P28230

Pathways: Cell-Cell Junction Organization