TDP2 antibody

Catalog No. ABIN6252692

This anti-TDP2 antibody is a Mouse Monoclonal antibody detecting TDP2 in WB, IF, FACS and IHC (p). Suitable for Human.

Quick Overview for TDP2 antibody (ABIN6252692)

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TDP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TDP2-1258

Product Details anti-TDP2 Antibody

Purification: Protein G affinity chromatography

Immunogen: Human recombinant full length protein was used as the immunogen for this TDP2 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TDP2 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TDP2

Target: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Alternative Name: TDP2

Background: This mAb recognizes a protein of 41  kDa, which is identified as TDP2, or ETS1 associated protein II. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.

Gene ID: 51567

UniProt: O95551