Glutamate Receptor Ionotropic, NMDA 2A (GRIN2A) (pTyr1325) antibody

Catalog No. ABIN6254089

This anti- antibody is a Rabbit Polyclonal antibody detecting in ELISA and WB. Suitable for Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Glutamate Receptor Ionotropic, NMDA 2A (GRIN2A) (pTyr1325) antibody (ABIN6254089)

Target: Glutamate Receptor Ionotropic, NMDA 2A (GRIN2A)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Binding Specificity: pTyr1325

Specificity: Specific for endogenous levels of the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Our NMDA NR2A Subunit (Tyr1325) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects NMDA NR2A Subunit (Tyr1325) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References

Somkuwar, Mandyam et al.: "Individual Differences in Ethanol Drinking and Seeking Behaviors in Rats Exposed to Chronic Intermittent Ethanol Vapor Exposure is Associated with Altered CaMKII Autophosphorylation in the Nucleus ..." in: Brain sciences, Vol. 9, Issue 12, (2019) (PubMed).

Takashima, Fannon, Galinato, Steiner, An, Zemljic-Harpf, Somkuwar, Head, Mandyam: "Neuroadaptations in the dentate gyrus following contextual cued reinstatement of methamphetamine seeking." in: Brain structure & function, Vol. 223, Issue 5, pp. 2197-2211, (2019) (PubMed).

Target Details

Target: Glutamate Receptor Ionotropic, NMDA 2A (GRIN2A)

Alternative Name: EPND

Background:

Synonyms: EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

Description Logic: Our NMDA NR2A Subunit (Tyr1325) rabbit polyclonal phosphospecific primary antibody is produced . It detects NMDA NR2A Subunit (Tyr1325) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: GRIN2A

Molecular Weight: 180 kDa

Gene ID: 24409

UniProt: Q00959