Abscisic Acid Insensitive 5 (ABI5) antibody

Catalog No. ABIN4966067

The Rabbit Polyclonal anti-Abscisic Acid Insensitive 5 (ABI5) antibody is suitable to detect Abscisic Acid Insensitive 5 (ABI5) in samples from Arabidopsis thaliana. It has been validated for IL and WB.

Quick Overview for Abscisic Acid Insensitive 5 (ABI5) antibody (ABIN4966067)

Target: Abscisic Acid Insensitive 5 (ABI5)

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Product Details

Purpose: Anti-ABI5 | Abscisic acid insensitive 5 (peptide antibody)

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Brassica napus,Populus trichocarpa

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence

Application Details

Application Notes: 1: 140 (IL), 1 : 200 (WB)

Comment:

ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as most sensitive detection reagents.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water to each tube

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: Abscisic Acid Insensitive 5 (ABI5)

Background:

Background: ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.

Additional information: MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here.

Molecular Weight: 47 kDa

Gene ID: 818199

UniProt: Q9SJN0