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Histone 3 antibody (H3K9ac)

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, IF, IHC, ELISA and ICC. It is suitable to detect Histone 3 in samples from Human, Mouse and Rat.
Catalog No. ABIN6254904

Quick Overview for Histone 3 antibody (H3K9ac) (ABIN6254904)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
  • Binding Specificity

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    H3K9ac

    Specificity

    Acetyl-Histone H3(Lys9) Antibody detects endogenous levels of Acetyl-Histone H3 only when phosphorylated at Lys9

    Cross-Reactivity

    Human, Mouse (Murine), Rat (Rattus)

    Purification

    Immunogen affinity purified

    Immunogen

    A synthesized peptide derived from human Acetyl-Histone H3 around the phosphorylation site of Lys9

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C.Stable for 12 months from date of receipt

    Expiry Date

    12 months
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Background

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

    Molecular Weight

    17kDa

    Gene ID

    8, 350, 835

    UniProt

    P68431, Q71DI3, P84243
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