Histone H3.1 antibody (pSer10)

Catalog No. ABIN6254919

There are 2+ publications for this product available. The Rabbit Polyclonal anti-Histone H3.1 antibody has been validated for WB, IF, ELISA, IHC and ICC. It is suitable to detect Histone H3.1 in samples from Human, Mouse and Rat.

Quick Overview for Histone H3.1 antibody (pSer10) (ABIN6254919)

Target: Histone H3.1 (HIST1H3B)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Histone H3.1 Antibody

Binding Specificity: pSer10

Specificity: Phospho-Histone H3.1 (Ser10) Antibody detects endogenous levels of Histone H3.1 only when phosphorylated at Serine 10

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Serine 10

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-Histone H3.1 antibody (ABIN6254919)

Liang, Sun, Zhao, Zhang, Gu, Dong, Zhang, Sun, Sun: "Rictor regulates the vasculogenic mimicry of melanoma via the AKT-MMP-2/9 pathway." in: Journal of cellular and molecular medicine, Vol. 21, Issue 12, pp. 3579-3591, (2018) (PubMed).

Wu, Wang, Yang, Huang, Hu, Shu, Zhang, Zheng: "MAD2-p31comet axis deficiency reduces cell proliferation, migration and sensitivity of microtubule-interfering agents in glioma." in: Biochemical and biophysical research communications, Vol. 498, Issue 1, pp. 157-163, (2018) (PubMed).

Target Details for Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Alternative Name: Histone H3.1

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Molecular Weight: 17kDa

Gene ID: 8, 350, 835

UniProt: P68431, Q71DI3, P84243