Histone H3.1 antibody (pSer10)

Catalog No. ABIN6254919

The Rabbit Polyclonal anti-Histone H3.1 antibody has been validated for WB, IHC, IF, ELISA and ICC. It is suitable to detect Histone H3.1 in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for Histone H3.1 antibody (pSer10) (ABIN6254919)

Target: Histone H3.1 (HIST1H3B)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-Histone H3.1 Antibody

Binding Specificity: pSer10

Specificity: Phospho-Histone H3.1 (Ser10) Antibody detects endogenous levels of Histone H3.1 only when phosphorylated at Serine 10

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Serine 10

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-Histone H3.1 antibody (ABIN6254919)

Liang, Sun, Zhao, Zhang, Gu, Dong, Zhang, Sun, Sun: "Rictor regulates the vasculogenic mimicry of melanoma via the AKT-MMP-2/9 pathway." in: Journal of cellular and molecular medicine, Vol. 21, Issue 12, pp. 3579-3591, (2018) (PubMed).

Wu, Wang, Yang, Huang, Hu, Shu, Zhang, Zheng: "MAD2-p31comet axis deficiency reduces cell proliferation, migration and sensitivity of microtubule-interfering agents in glioma." in: Biochemical and biophysical research communications, Vol. 498, Issue 1, pp. 157-163, (2018) (PubMed).

Target Details for Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Alternative Name: Histone H3.1

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Molecular Weight: 17kDa

Gene ID: 8, 350, 835

UniProt: P68431, Q71DI3, P84243