CPI-17 antibody (pThr38)

Catalog No. ABIN6256551

The Rabbit Polyclonal anti-CPI-17 antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect CPI-17 in samples from Human, Mouse and Rat.

Quick Overview for CPI-17 antibody (pThr38) (ABIN6256551)

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPI-17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CPI-17 Antibody

Binding Specificity: pThr38

Specificity: Phospho-CPI17alpha (Thr38) Antibody detects endogenous levels of CPI17alpha only when phosphorylated at Threonine 38.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CPI17alpha around the phosphorylation site of Thr38.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CPI-17

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Alternative Name: PPP1R14A

Background:

Description: Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

Gene: PPP1R14A

Molecular Weight: 22kDa

Gene ID: 94274

UniProt: Q96A00