Cytochrome P450 3A4, 3A5 antibody

Catalog No. ABIN6257328

The Rabbit Polyclonal anti-not_set antibody has been validated for WB, IF, ELISA and ICC. It is suitable to detect not_set in samples from Human, Mouse and Rat.

Quick Overview for Cytochrome P450 3A4, 3A5 antibody (ABIN6257328)

Target: Cytochrome P450 3A4, 3A5

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details

Specificity: Cytochrome P450 3A4/5 Antibody detects endogenous levels of total Cytochrome P450 3A4/5

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotype: IgG

Application Details

Application Notes: WB 1:500~1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details

Target: Cytochrome P450 3A4, 3A5

Alternative Name: Cytochrome P450 3A4/5

Background:

Description: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599).

Gene: CYP3A4

Molecular Weight: 57 kDa

Gene ID: 1576

UniProt: P08684, P20815