GNAT1 antibody (Internal Region)

Catalog No. ABIN6257751

The Rabbit Polyclonal anti-GNAT1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect GNAT1 in samples from Human, Mouse and Rat.

Quick Overview for GNAT1 antibody (Internal Region) (ABIN6257751)

Target: GNAT1 (Guanine Nucleotide Binding Protein (G Protein), alpha Transducing Activity Polypeptide 1 (GNAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GNAT1 Antibody

Binding Specificity: Internal Region

Specificity: GNAT1 Antibody detects endogenous levels of total GNAT1.

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GNAT1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GNAT1

Target: GNAT1 (Guanine Nucleotide Binding Protein (G Protein), alpha Transducing Activity Polypeptide 1 (GNAT1))

Alternative Name: GNAT1

Background:

Description: Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (PubMed:22190596). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).

Gene: GNAT1

Molecular Weight: 36 kDa

Gene ID: 2779

UniProt: P11488

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Phototransduction