CROT antibody (C-Term)

Catalog No. ABIN625820

The Goat Polyclonal anti-CROT antibody has been validated for WB, ELISA and IHC. It is suitable to detect CROT in samples from Human and Rat.

Quick Overview for CROT antibody (C-Term) (ABIN625820)

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CROT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CROT Antibody

Binding Specificity: C-Term

Purpose: Crot (rat)

Sequence: RSCLETDAEK LVE

Cross-Reactivity: Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: RSCLETDAEKLVE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx 75 kDa band observed in Rat Kidney lysates (calculated MW of 70.3 kDa according to NP_114193.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CROT

Target: CROT (Carnitine O-Octanoyltransferase (CROT))

Alternative Name: Crot

Background: Crot, carnitine O-octanoyltransferase, COT, peroxisomal carnitine O-octanoyltransferase

Gene ID: 83842

NCBI Accession: NP_114193

Pathways: Monocarboxylic Acid Catabolic Process