Aggrecan antibody (N-Term)

Catalog No. ABIN625841

The Rabbit Polyclonal anti-Aggrecan antibody has been validated for IHC and IHC (p). It is suitable to detect Aggrecan in samples from Human, Pig, Hamster and Monkey.

Quick Overview for Aggrecan antibody (N-Term) (ABIN625841)

Target: Aggrecan (ACAN)

Reactivity: Human, Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aggrecan Antibody

Binding Specificity: N-Term

Specificity: Human ACAN / Aggrecan. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NCAN (56 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Hamster, Pig (100%) Mouse, Rat, Bovine, Panda, Horse (94%) Dog, Elephant (89%) Bat (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from N-terminus of human ACAN / Aggrecan. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Hamster, Pig (100%), Mouse, Rat, Bovine, Panda, Horse (94%), Dog, Elephant (89%), Bat (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (7 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 7 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan

Background: Name/Gene ID: ACAN

Synonyms: ACAN, AGCAN, Aggrecan core protein, Acg1, Aggrecan, CSPG1, CSPGCP, CSPCP, AGC1, Aggrecan 1, Large aggregating proteoglycan, MSK16, SEDK, MCSPG

Gene ID: 176

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport