SPIC antibody (Internal Region)

Catalog No. ABIN6258474

The Rabbit Polyclonal anti-SPIC antibody has been validated for ELISA, WB, IF and ICC. It is suitable to detect SPIC in samples from Human and Mouse.

Quick Overview for SPIC antibody (Internal Region) (ABIN6258474)

Target: SPIC (Spi-C Transcription Factor (Spi-1/PU.1 Related) (SPIC))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPIC antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SPIC Antibody

Binding Specificity: Internal Region

Specificity: SPIC Antibody detects endogenous levels of total SPIC.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SPIC, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SPIC

Target: SPIC (Spi-C Transcription Factor (Spi-1/PU.1 Related) (SPIC))

Alternative Name: SPIC

Background:

Description: Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis. Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression (By similarity).

Gene: SPIC

Molecular Weight: 29 kDa

Gene ID: 121599

UniProt: Q8N5J4