ATP5H antibody (C-Term)

Catalog No. ABIN6258536

This Rabbit Polyclonal antibody specifically detects ATP5H in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATP5H antibody (C-Term) (ABIN6258536)

Target: ATP5H (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATP5H Antibody

Binding Specificity: C-Term

Specificity: ATP5H Antibody detects endogenous levels of total ATP5H.

Predicted Reactivity: Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATP5H, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP5H

Target: ATP5H (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H))

Alternative Name: ATP5H

Background:

Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.

Gene: ATP5H

Molecular Weight: 26 kDa

Gene ID: 10476

UniProt: O75947

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process