LYAR antibody (Internal Region)

Catalog No. ABIN6259573

This anti-LYAR antibody is a Rabbit Polyclonal antibody detecting LYAR in WB, ELISA, ICC and IF. Suitable for Human.

Quick Overview for LYAR antibody (Internal Region) (ABIN6259573)

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-LYAR Antibody

Binding Specificity: Internal Region

Specificity: LYAR Antibody detects endogenous levels of total LYAR.

Predicted Reactivity: Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LYAR, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LYAR

Target: LYAR (Ly1 Antibody Reactive (LYAR))

Alternative Name: LYAR

Target Type: Antibody

Background:

Description: Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed:28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed:25092918). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).

Gene: LYAR

Molecular Weight: 44 kDa

Gene ID: 55646

UniProt: Q9NX58