alpha 2 Macroglobulin antibody (Internal Region)

Catalog No. ABIN6259666

This anti-alpha 2 Macroglobulin antibody is a Rabbit Polyclonal antibody detecting alpha 2 Macroglobulin in WB and ELISA. Suitable for Human, Rat and Mouse.

Quick Overview for alpha 2 Macroglobulin antibody (Internal Region) (ABIN6259666)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: Internal Region

Specificity: Alpha 2 Macroglobulin/A2M Antibody detects endogenous levels of total alpha 2 Macroglobulin/A2M.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human alpha 2 Macroglobulin/A2M, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: A2M

Background:

Description: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Gene: A2M

Molecular Weight: 180 kDa

Gene ID: 2

UniProt: P01023

Pathways: Lipid Metabolism