CREB3L1 antibody (N-Term)

Catalog No. ABIN6261026

This Rabbit Polyclonal antibody specifically detects CREB3L1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CREB3L1 antibody (N-Term) (ABIN6261026)

Target: CREB3L1 (CAMP Responsive Element Binding Protein 3-Like 1 (CREB3L1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB3L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CREB3L1 Antibody

Binding Specificity: N-Term

Specificity: CREB3L1,OASIS Antibody detects endogenous levels of total CREB3L1,OASIS.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CREB3L1,OASIS, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CREB3L1

Target: CREB3L1 (CAMP Responsive Element Binding Protein 3-Like 1 (CREB3L1))

Alternative Name: CREB3L1

Background:

Description: Transcription factor involved in unfolded protein response (UPR). Binds the DNA consensus sequence 5'-GTGXGCXGC-3' (PubMed:21767813). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. Directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. Does not regulate COL1A1 in other tissues, such as skin (By similarity). Required to protect astrocytes from ER stress-induced cell death. In astrocytes, binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation (By similarity). Required for TGFB1 to activate genes involved in the assembly of collagen extracellular matrix (PubMed:25310401).

Gene: CREB3L1

Molecular Weight: 55-58 kDa and 84 kDa

Gene ID: 90993

UniProt: Q96BA8

Pathways: Thyroid Hormone Synthesis, Chromatin Binding