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Histone 3 antibody (N-Term)

This anti-Histone 3 antibody is a Rabbit Polyclonal antibody detecting Histone 3 in WB, IF, IHC, ELISA, ChIP, IP and ICC. Suitable for Human, Mouse and Rat.
Catalog No. ABIN6262270

Quick Overview for Histone 3 antibody (N-Term) (ABIN6262270)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

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    N-Term

    Specificity

    Histone H3 Antibody detects endogenous levels of total Histone H3.

    Predicted Reactivity

    Horse,Sheep,Rabbit

    Purification

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    Immunogen

    A synthesized peptide derived from human Histone H3, corresponding to a region within N-terminal amino acids.

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C. Stable for 12 months from date of receipt.

    Expiry Date

    12 months
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    HIST3H3

    Background

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST3H3

    Molecular Weight

    15kDa

    Gene ID

    8290

    UniProt

    Q16695
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