MIA3 antibody (C-Term)

Catalog No. ABIN6263247

The Rabbit Polyclonal anti-MIA3 antibody has been validated for ELISA and WB. It is suitable to detect MIA3 in samples from Human, Mouse and Rat.

Quick Overview for MIA3 antibody (C-Term) (ABIN6263247)

Target: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIA3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MIA3 Antibody

Binding Specificity: C-Term

Specificity: MIA3 Antibody detects endogenous levels of total MIA3.

Predicted Reactivity: Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MIA3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MIA3

Target: MIA3 (Melanoma Inhibitory Activity Family, Member 3 (MIA3))

Alternative Name: MIA3

Background:

Description: Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255).

Gene: MIA3

Molecular Weight: 214kDa

Gene ID: 375056

UniProt: Q5JRA6