ZC3HC1 antibody (C-Term)

Catalog No. ABIN6263640

The Rabbit Polyclonal anti-ZC3HC1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect ZC3HC1 in samples from Human.

Quick Overview for ZC3HC1 antibody (C-Term) (ABIN6263640)

Target: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZC3HC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ZC3HC1 Antibody

Binding Specificity: C-Term

Specificity: NIPA Antibody detects endogenous levels of total NIPA.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NIPA, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ZC3HC1

Target: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Alternative Name: ZC3HC1

Background:

Description: Essential component of a SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.

Gene: ZC3HC1

Molecular Weight: 55kDa

Gene ID: 51530

UniProt: Q86WB0