SETMAR antibody (N-Term)

Catalog No. ABIN6265021

This anti-SETMAR antibody is a Rabbit Polyclonal antibody detecting SETMAR in ELISA and WB. Suitable for Human.

Quick Overview for SETMAR antibody (N-Term) (ABIN6265021)

Target: SETMAR (SET Domain and Mariner Transposase Fusion Gene (SETMAR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETMAR antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-SETMAR Antibody

Binding Specificity: N-Term

Specificity: SETMAR Antibody detects endogenous levels of total SETMAR.

Predicted Reactivity: Bovine,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SETMAR, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SETMAR

Target: SETMAR (SET Domain and Mariner Transposase Fusion Gene (SETMAR))

Alternative Name: SETMAR

Background:

Description: Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity (PubMed:16332963, PubMed:16672366, PubMed:17877369, PubMed:17403897, PubMed:18263876, PubMed:22231448, PubMed:24573677, PubMed:20521842). In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining (PubMed:16332963, PubMed:21187428, PubMed:22231448). Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A (PubMed:18790802, PubMed:20457750).

Gene: SETMAR

Molecular Weight: 78 kDa

Gene ID: 6419

UniProt: Q53H47

Pathways: Positive Regulation of Response to DNA Damage Stimulus