TRAM2 antibody (C-Term)

Catalog No. ABIN6265712

This Rabbit Polyclonal antibody specifically detects TRAM2 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TRAM2 antibody (C-Term) (ABIN6265712)

Target: TRAM2 (Translocation Associated Membrane Protein 2 (TRAM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TRAM2 Antibody

Binding Specificity: C-Term

Specificity: TRAM2 Antibody detects endogenous levels of total TRAM2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TRAM2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TRAM2

Target: TRAM2 (Translocation Associated Membrane Protein 2 (TRAM2))

Alternative Name: TRAM2

Background:

Description: Necessary for collagen type I synthesis. May couple the activity of the ER Ca2+ pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca2+ concentration at the site of collagen synthesis, and a high Ca2+ concentration may be necessary for the function of molecular chaperones involved in collagen folding. Required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, may act as a ceramide sensor for regulated alternative translocation (RAT) (PubMed:27499293).

Gene: TRAM2

Molecular Weight: 43 kDa

Gene ID: 9697

UniProt: Q15035