ATP6V0D1 antibody (Internal Region)

Catalog No. ABIN6265939

This anti-ATP6V0D1 antibody is a Rabbit Polyclonal antibody detecting ATP6V0D1 in WB, ELISA, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for ATP6V0D1 antibody (Internal Region) (ABIN6265939)

Target: ATP6V0D1 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D1 (ATP6V0D1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATP6V0D1 Antibody

Binding Specificity: Internal Region

Specificity: ATP6V0D1 Antibody detects endogenous levels of total ATP6V0D1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATP6V0D1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP6V0D1

Target: ATP6V0D1 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D1 (ATP6V0D1))

Alternative Name: ATP6V0D1

Background:

Description: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).

Gene: ATP6V0D1

Molecular Weight: 40 kDa

Gene ID: 9114

UniProt: P61421

Pathways: Transition Metal Ion Homeostasis, Proton Transport, ER-Nucleus Signaling, Unfolded Protein Response