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Histone 3 antibody (H3K27me)

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IHC, IF, ELISA and ICC. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.
Catalog No. ABIN6266165
$494.77
Plus shipping costs $50.00
Shipping to: United States
Delivery in 11 to 12 Business Days

Quick Overview for Histone 3 antibody (H3K27me) (ABIN6266165)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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  • 1145
  • 1017
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Human, Mouse

Host

  • 1454
  • 254
  • 8
  • 5
Rabbit

Clonality

  • 1114
  • 606
  • 1
Polyclonal

Conjugate

  • 900
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  • 67
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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)
  • Binding Specificity

    • 72
    • 51
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    • 39
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    • 33
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    H3K27me

    Specificity

    Mono-Methyl-Histone H3 (Lys27)/H3K27me1 Antibody detects endogenous levels of Mono-Methyl-Histone H3 only when methylated at Lys27.

    Purification

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    Immunogen

    A synthetic methylated peptide derived from human Mono-Methyl-Histone H3 around the methylation site of Lys27.

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C. Stable for 12 months from date of receipt.

    Expiry Date

    12 months
  • Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, (2017) (PubMed).

  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    HIST3H3

    Background

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST3H3

    Molecular Weight

    15kDa

    Gene ID

    8290

    UniProt

    Q16695
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