For best experience we recommend to activate Javascript in your browser.

Histone 3 antibody (H3K27me2)

Name: Histone 3 antibody (H3K27me2)
SKU: ABIN6266166
Price: 494.77 USD
Availability: InStock

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, IHC, IF, ELISA, IP and ICC. It is suitable to detect Histone 3 in samples from Human, Mouse and Rat. There is 1 publication available.

Western blot analysis of extracts of HeLa using H3K27me2 antibody. (Histone 3 antibody (H3K27me2))

IF/ICC

ABIN6277199 at 1/100 staining Rat heart tissue by IHC-P. (Histone 3 antibody (H3K27me2))

IHC

3 images

(1 reference)

Catalog No. ABIN6266166

Quick Overview for Histone 3 antibody (H3K27me2) (ABIN6266166)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Reactivity

1429
937
838
39
39
37
34
32
25
23
20
19
11
6
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1078
389
387
339
248
191
188
172
163
158
145
129
113
48
48
37
35
26
25
15
14
7
7
7
4
3
1
1
1
1

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Discover our top product H3 Primary Antibody

Binding Specificity
51

50

49

45

42

40

38

38

36

36

35

34

32

32

32

30

29

26

24

23

22

19

19

18

18

18

17

17

15

15

15

15

15

14

14

13

12

12

12

12

12

11

11

10

10

9

9

9

8

8
H3K27me2

Specificity

Di-Methyl-Histone H3 (di methyl Lys27)/H3K27me2 Antibody detects endogenous levels of Di-Methyl-Histone H3 only when methylated at di methyl Lys27.

Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen

A synthetic methylated peptide derived from human Di-Methyl-Histone H3 around the methylation site of di methyl Lys27.

Isotype

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date

12 months
Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, (2017) (PubMed).
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

HIST3H3

Background

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene: HIST3H3

Molecular Weight

15kDa

Gene ID

8290

UniProt

Q16695