Histone 3 antibody (H3K27me3)
(1 reference)-
- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- H3K27me3
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- Tri-Methyl-Histone H3 (Lys27)/H3K27me3 Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys27.
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthetic methylated peptide derived from human Tri-Methyl-Histone H3 around the methylation site of Lys27.
- Isotype
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 Reactivity: Human WB, IF, IHC, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 Reactivity: Human WB, IF, IHC, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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: "HBx-mediated decrease of AIM2 contributes to hepatocellular carcinoma metastasis." in: Molecular oncology, Vol. 11, Issue 9, pp. 1225-1240, (2018) (PubMed).
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: "HBx-mediated decrease of AIM2 contributes to hepatocellular carcinoma metastasis." in: Molecular oncology, Vol. 11, Issue 9, pp. 1225-1240, (2018) (PubMed).
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- Target
- Histone 3 (H3)
- Alternative Name
- HIST3H3 (H3 Products)
- Background
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Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene: HIST3H3
- Molecular Weight
- 15kDa
- Gene ID
- 8290
- UniProt
- Q16695
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