Histone 3 antibody (H3K4me3)

Catalog No. ABIN6266172

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, IF, IHC, ELISA, ChIP, IP and ICC. It is suitable to detect Histone 3 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for Histone 3 antibody (H3K4me3) (ABIN6266172)

Key Features:

• High quality Histone 3 Primary Antibody for the detection of H3.
• Reliable product with high standard validation data

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K4me3

Specificity: Tri-Methyl-Histone H3 (Lys4)/H3K4me3 Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys4.

Predicted Reactivity: Horse,Sheep,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthetic methylated peptide derived from human Tri-Methyl-Histone H3 around the methylation site of Lys4.

Isotype: IgG

Product Specific Information:

What can the Histone 3 Antibody ABIN6266172 be used for?: This polyclonal Histone 3 Antibody detects Histone 3. The Histone 3 Antibody has been validated for various applications and can be used for the detection of Histone 3 and derivatives by Western Blotting, Immunofluorescence, Immunohistochemistry, ELISA, Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry.

What validation data is available for this Histone 3 Antibody?: The primary antibody is referenced in 1 publications and is characterized by a proven, very high reliability. It has currently 3 product images that show its performance in a variety of applications. The product is currently available in 100 μL, 200 μL quantities. Histone 3 Antibody for the detection of Histone 3 and derivatives.

What is the function of Histone 3?: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails\; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008].

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Histone 3 antibody (ABIN6266172)

Yi, Guo, Guo, Sun, Yang, Wang, Li, Xie, Cai, Wang: "EZH2-mediated epigenetic silencing of TIMP2 promotes ovarian cancer migration and invasion." in: Scientific reports, Vol. 7, Issue 1, pp. 3568, (2017) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: HIST3H3

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST3H3

Molecular Weight: 15kDa

Gene ID: 8290

UniProt: Q16695