Histone 3 antibody (H3K79me)
Quick Overview for Histone 3 antibody (H3K79me) (ABIN6266173)
Target
See all Histone 3 (H3) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- H3K79me
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Specificity
- Mono-Methyl-Histone H3 (Lys79)/H3K79me1 Antibody detects endogenous levels of Mono-Methyl-Histone H3 only when methylated at Lys79.
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Predicted Reactivity
- Horse,Sheep,Rabbit
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Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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Immunogen
- A synthetic methylated peptide derived from human Mono-Methyl-Histone H3 around the methylation site of Lys79.
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Isotype
- IgG
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Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
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Expiry Date
- 12 months
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- Histone 3 (H3) (Histone H3 (H3))
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Alternative Name
- HIST3H3
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Background
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Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene: HIST3H3
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Molecular Weight
- 15kDa
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Gene ID
- 8290
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UniProt
- Q16695
Target
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