Histone 3 antibody (H3K9me2)

Catalog No. ABIN6266177

This Rabbit Polyclonal antibody specifically detects Histone 3 in WB, IF, IHC, ELISA, ChIP, IP and ICC. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for Histone 3 antibody (H3K9me2) (ABIN6266177)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K9me2

Specificity: Di-Methyl-Histone H3 (Lys9)/H3K9me2 Antibody detects endogenous levels of Di-Methyl-Histone H3 only when methylated at Lys9.

Predicted Reactivity: Horse,Sheep,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthetic methylated peptide derived from human Di-Methyl-Histone H3 around the methylation site of Lys9.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Histone 3 antibody (ABIN6266177)

Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, (2017) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: HIST3H3

Background:

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: HIST3H3

Molecular Weight: 15kDa

Gene ID: 8290

UniProt: Q16695