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Histone 3 antibody (H3K79me2)

The Rabbit Polyclonal anti-Histone 3 antibody has been validated for WB, IHC, IF, ELISA, ChIP, IP and ICC. It is suitable to detect Histone 3 in samples from Human and Mouse.
Catalog No. ABIN6266346

Quick Overview for Histone 3 antibody (H3K79me2) (ABIN6266346)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

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Human, Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Histone 3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Binding Specificity

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    H3K79me2

    Specificity

    Di-Methyl-Histone H3 (Lys79)/H3K79me2 Antibody detects endogenous levels of Di-Methyl-Histone H3 only when methylated at Lys79.

    Predicted Reactivity

    Horse,Sheep,Rabbit

    Purification

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    Immunogen

    A synthetic methylated peptide derived from human Di-Methyl-Histone H3 around the methylation site of Lys79.

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C. Stable for 12 months from date of receipt.

    Expiry Date

    12 months
  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    HIST3H3

    Background

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST3H3

    Molecular Weight

    16kDa

    Gene ID

    8290

    UniProt

    Q16695
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