Cytochrome C antibody (AA 1-80)

Catalog No. ABIN6294256

This Mouse Monoclonal antibody specifically detects Cytochrome C in WB, IHC, FACS and IF. It exhibits reactivity toward Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster and Frog.

Quick Overview for Cytochrome C antibody (AA 1-80) (ABIN6294256)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Cytochrome C Antibody

Binding Specificity: AA 1-80

Purpose: Mouse anti-Human/Mouse/Rat/Horse/Dog/Pigeon/Frog/Drosophila Cytochrome C Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.

Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome c

Background:

Target Description: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Gene Symbol: CYCS///CYC

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity