Myogenin antibody (AA 138-158)

Catalog No. ABIN6294312

This anti-Myogenin antibody is a Mouse Monoclonal antibody detecting Myogenin in WB, ELISA, IHC, IF, FACS and IP. Suitable for Human, Mouse, Rat, Pig, Cat and Dog.

Quick Overview for Myogenin antibody (AA 138-158) (ABIN6294312)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivity: Human, Mouse, Rat, Pig, Cat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myogenin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-Myogenin Antibody

Binding Specificity: AA 138-158

Purpose: Mouse anti-Human/Mouse/Rat/Dog/Cat/Pig Myogenin Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.

Immunogen: Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.

Application Details

Application Notes: ELISA: order BSA-free format
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: Myogenin

Background:

Target Description: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Gene Symbol: MYOG///BHLHC3///MYF4

Gene ID: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development