CD79a antibody (AA 202-216)

Catalog No. ABIN6294392

The Mouse Monoclonal anti-CD79a antibody has been validated for FACS, IF, WB and IHC. It is suitable to detect CD79a in samples from Human, Mouse, Rat, Cow, Pig and Monkey.

Quick Overview for CD79a antibody (AA 202-216) (ABIN6294392)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD79a antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD79a Antibody

Binding Specificity: AA 202-216

Purpose: Mouse anti-Human/Mouse/Rat/Monkey/Pig/Cow CD79a Antibody [Sodium Azide Free]

Specificity: Cell surface, cytoplasmic

Purification: Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for the CD79a antibody.

Immunogen: Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for the CD79a antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT
WB: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Alternative Name: B-cell antigen receptor complex-associated protein alpha chain

Background:

Target Description: CD79a, also known as B-cell antigen receptor complex-associated protein alpha chain and MB-1 membrane glycoprotein, is a protein that in humans is encoded by the CD79A gene. The CD79a protein together with the related CD79b protein, forms a dimer associated with membrane-bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR). This occurs in a similar manner to the association of CD3 with the T-cell receptor, and enables the cell to respond to the presence of antigens on its surface.CD79a plays multiple and diverse roles in B cell development and function. The CD79a/b heterodimer associates non-covalently with the immunoglobulin heavy chain through its transmembrane region, thus forming the BCR along with the immunoglobulin light chain and the pre-BCR when associated with the surrogate light chain in developing B cells. Association of the CD79a/b heterodimer with the immunoglobulin heavy chain is required for surface expression of the BCR and BCR induced calcium flux and protein tyrosine phosphorylation. Genetic deletion of the transmembrane exon of CD79A results in loss of CD79a protein and a complete block of B cell development at the pro to pre B cell transition. Similarly, humans with homozygous splice variants in CD79A predicted to result in loss of the transmembrane region and a truncated or absent protein display agammaglobulinemia and no peripheral B cells. [Wiki]

Gene Symbol: CD79A///IGA///MB1

Gene ID: 973

UniProt: P11912

Pathways: BCR Signaling