p63 antibody (AA 5-17)

Catalog No. ABIN6294546

This anti-p63 antibody is a Rabbit Polyclonal antibody detecting p63 in WB, IHC, IF and FACS. Suitable for Human, Mouse, Rat and Cow.

Quick Overview for p63 antibody (AA 5-17) (ABIN6294546)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p63 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-p63 Antibody

Binding Specificity: AA 5-17

Purpose: Rabbit anti-Human/Mouse/Rat/Cow p40 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Amino acids ENNAQTQFSEPQY (aa 5-17) of human p40 / p63 delta were used as the immunogen for this antibody.

Immunogen: Amino acids ENNAQTQFSEPQY (aa 5-17) of human p40 / p63 delta were used as the immunogen for this antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10e6 cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Alternative Name: Tumor protein 63

Background:

Target Description: p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p40 antibody 's ability to distinguish between the tumor types appears to be superior when compared to p63. The ability to utilize a p40 antibody probe as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Gene Symbol: TP63///KET///P63///P73H///P73L///TP73L

Gene ID: 8626

UniProt: Q9H3D4