Desmocollin 2 antibody (Extracellular Domain)

Catalog No. ABIN6295454

The Mouse Monoclonal anti-Desmocollin 2 antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect Desmocollin 2 in samples from Human, Mouse and Rat.

Quick Overview for Desmocollin 2 antibody (Extracellular Domain) (ABIN6295454)

Target: Desmocollin 2 (DSC2)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmocollin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Desmocollin 2 Antibody

Binding Specificity: Extracellular Domain

Purpose: Mouse anti-Human/Mouse/Rat Desmocollin 2/3 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: A recombinant protein corresponding to the extracellular domain of human Desmocollin 2 was used as the immunogen for the Desmocollin 2/3 antibody.

Immunogen: A recombinant protein corresponding to the extracellular domain of human Desmocollin 2 was used as the immunogen for the Desmocollin 2/3 antibody.

Application Details

Application Notes: WB: 0.5-1 μg/mL
FACS: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Desmocollin 2

Target: Desmocollin 2 (DSC2)

Alternative Name: Desmocollin-2

Background:

Target Description: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of Desmocollin-2 varies in the basal and suprabasal layers. And Desmocollin-3 is expressed more evenly throughout the suprabasal layers.

Gene Symbol: DSC2///CDHF2///DSC3

Gene ID: 1824

UniProt: Q02487