FOXP3 antibody (N-Term)

Catalog No. ABIN6295493

The Mouse Monoclonal anti-FOXP3 antibody has been validated for FACS, IHC and IF. It is suitable to detect FOXP3 in samples from Human and Mouse.

Quick Overview for FOXP3 antibody (N-Term) (ABIN6295493)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FOXP3 Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-Human/Mouse FOXP3 Antibody [Sodium Azide Free]

Specificity: Predominantly nuclear, some cytoplasmic

Purification: An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

Immunogen: An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Forkhead box protein P3

Background:

Target Description: The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Gene Symbol: FOXP3///IPEX///JM2

Gene ID: 50943

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation