CD81 antibody

Catalog No. ABIN6295927

This Mouse Monoclonal antibody specifically detects CD81 in FACS, WB, IHC, IF and Func. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD81 antibody (ABIN6295927)

Target: CD81

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func)

Product Details anti-CD81 Antibody

Purpose: Mouse anti-Human/Mouse/Rat CD81 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic, membranous

Purification: A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the CD81 antibody.

Immunogen: A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the CD81 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
Functional Studies: order BSA free formulation
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD81

Target: CD81

Alternative Name: CD81 antigen

Background:

Target Description: This mAb recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Gene Symbol: CD81///TAPA1///TSPAN28

Gene ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C